Composite

Part:BBa_K3504022:Design

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-10-25)


Alphavirus replicon NSPs- SFV


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2766
    Illegal EcoRI site found at 4344
    Illegal XbaI site found at 7239
    Illegal PstI site found at 1018
    Illegal PstI site found at 3378
    Illegal PstI site found at 4392
    Illegal PstI site found at 5045
    Illegal PstI site found at 5110
    Illegal PstI site found at 6970
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2766
    Illegal EcoRI site found at 4344
    Illegal NheI site found at 1224
    Illegal NheI site found at 3065
    Illegal NheI site found at 6732
    Illegal PstI site found at 1018
    Illegal PstI site found at 3378
    Illegal PstI site found at 4392
    Illegal PstI site found at 5045
    Illegal PstI site found at 5110
    Illegal PstI site found at 6970
    Illegal NotI site found at 6736
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2766
    Illegal EcoRI site found at 4344
    Illegal BglII site found at 7314
    Illegal XhoI site found at 5897
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2766
    Illegal EcoRI site found at 4344
    Illegal XbaI site found at 7239
    Illegal PstI site found at 1018
    Illegal PstI site found at 3378
    Illegal PstI site found at 4392
    Illegal PstI site found at 5045
    Illegal PstI site found at 5110
    Illegal PstI site found at 6970
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2766
    Illegal EcoRI site found at 4344
    Illegal XbaI site found at 7239
    Illegal PstI site found at 1018
    Illegal PstI site found at 3378
    Illegal PstI site found at 4392
    Illegal PstI site found at 5045
    Illegal PstI site found at 5110
    Illegal PstI site found at 6970
    Illegal NgoMIV site found at 2211
    Illegal NgoMIV site found at 2448
    Illegal NgoMIV site found at 3558
    Illegal NgoMIV site found at 4295
    Illegal NgoMIV site found at 4305
    Illegal NgoMIV site found at 5971
    Illegal AgeI site found at 5965
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3115
    Illegal BsaI.rc site found at 4598
    Illegal BsaI.rc site found at 7229


Design Notes

IDT


Source

NA

References